LOCUS       40924_9121        5756 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Plant expression vector pCB302 LIC GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Zurcher E, Liu J, di Donato M, Geisler M, Muller B.
  TITLE     Plant development regulated by cytokinin sinks
  JOURNAL   Science 353 (6303), 1027-1030 (2016)
  PUBMED    27701112
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   Muller B.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2016) Plant and Microbial Biology, University of 
            Zurich, Zollikerstrasse 107, Zurich, ZH 8008, Switzerland
REFERENCE   3  (bases 1 to 5756)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5756)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2016"; volume: "353"; issue: "6303"; pages: "1027-1030"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-JUL-2016) Plant and Microbial Biology, University of Zurich, 
            Zollikerstrasse 107, Zurich, ZH 8008, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(225..249)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        406..589
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             589..1137
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     terminator      1172..1424
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_recomb     1433..1462
                     /note="LIC; ligation-independent cloning (LIC) site"
     CDS             1466..2182
                     /label=EGFP
                     /note="enhanced GFP"
     primer_bind     complement(2213..2229)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2294..2318
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(2473..3618)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     CDS             complement(3920..4711)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(4923..5554)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    5632..5656
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     5737..5753
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agctttagcc gacccagacg agccaaggga tctttttgga atgctgctcc gtcgtcaggc
       61 tttccgacgt ttgggtggtt gaacagaagt cattatcgta cggaatgcca agcactcccg
      121 aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
      181 ccttttaaat atccgttatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat
      241 atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg atcatgagcg
      301 gagaattaag ggagtcacgt tatgaccccc gccgatgacg cgggacaagc cgttttacgt
      361 ttggaactga cagaaccgca acgattgaag gagccactca gccgcgggtt tctggagttt
      421 aatgagctaa gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca
      481 ctatcagcta gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct
      541 cggtatccaa ttagagtctc atattcactc tcaatccaaa taatctgcat gagcccagaa
      601 cgacgcccgg ccgacatccg ccgtgccacc gaggcggaca tgccggcggt ctgcaccatc
      661 gtcaaccact acatcgagac aagcacggtc aacttccgta ccgagccgca ggaaccgcag
      721 gagtggacgg acgacctcgt ccgtctgcgg gagcgctatc cctggctcgt cgccgaggtg
      781 gacggcgagg tcgccggcat cgcctacgcg ggcccctgga aggcacgcaa cgcctacgac
      841 tggacggccg agtcgaccgt gtacgtctcc ccccgccacc agcggacggg actgggctcc
      901 acgctctaca cccacctgct gaagtccctg gaggcacagg gcttcaagag cgtggtcgct
      961 gtcatcgggc tgcccaacga cccgagcgtg cgcatgcacg aggcgctcgg atatgccccc
     1021 cgcggcatgc tgcgggcggc cggcttcaag cacgggaact ggcatgacgt gggtttctgg
     1081 cagctggact tcagcctgcc ggtaccgccc cgtccggtcc tgcccgtcac cgagatctga
     1141 tgacccctag aggatcagct cgaatttccc cgatcgttca aacatttggc aataaagttt
     1201 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     1261 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     1321 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     1381 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tctagttgga
     1441 atgggttaac ccaactccaa tagccatggt gagcaagggc gaggagctgt tcaccggggt
     1501 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1561 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1621 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     1681 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1741 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1801 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1861 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1921 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     1981 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     2041 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     2101 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactca
     2161 cggcatggac gagctgtaca agtaaagcgg ccgcccgggc tgcagcccgg gggatccact
     2221 agttctagag cggccgccac cgcggtggag ctcagattgt cgtttcccgc cttcagttta
     2281 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     2341 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     2401 catgccaacc acaggagatc tcagtaaagc gctggctgaa cccccagccg gaactgaccc
     2461 cacaaggccc tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag
     2521 gccgctgcct cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg
     2581 ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg
     2641 gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc
     2701 ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag
     2761 gacctggcaa cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga
     2821 caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg
     2881 cgacaggcat tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg
     2941 gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc
     3001 caacacctgc tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt
     3061 gatcttcacg tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat
     3121 tttcttgttg cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat
     3181 cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt
     3241 cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc
     3301 ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc
     3361 caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg
     3421 cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac
     3481 catcgagccg acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat
     3541 ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg
     3601 gtcaaacgtc cgattcattc accctccttg cgggattgcc ccgactcacg ccggggcaat
     3661 gtgcccttat tcctgatttg acccgcctgg tgccttggtg tccagataat ccaccttatc
     3721 ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg tattccgaat
     3781 cttgccctgc acgaatacca gcgacccctt gcccaaatac ttgccgtggg cctcggcctg
     3841 agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt
     3901 gcgccacatc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
     3961 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
     4021 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
     4081 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
     4141 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
     4201 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
     4261 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
     4321 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
     4381 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
     4441 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
     4501 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt
     4561 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
     4621 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
     4681 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
     4741 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
     4801 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
     4861 taacatagta tcgacggagc cgattttgaa accacaatta tgggtgatgc tgccaactcg
     4921 agagcgggcc gggagggttc gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc
     4981 gcacagggcg cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta
     5041 aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct
     5101 gcctgtggac agcccctcaa atgtcaatag gtgcgcccct catctgtcag cactctgccc
     5161 ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata
     5221 ccgcagggca cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca
     5281 ggcgttttcg ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg
     5341 cccctcatct gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc
     5401 tcatctgtca gtgagggcca agttttccgc gaggtatcca caacgccggc ggccgcggtg
     5461 tctcgcacac ggcttcgacg gcgtttctgg cgcgtttgca gggccataga cggccgccag
     5521 cccagcggcg agggcaacca gcccggtgag cgtctagtgg actgatgggc tgcctgtatc
     5581 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
     5641 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt
     5701 aatgtactgg ggtggttttg gtaccgggcc ccccctcgag gtcgacggta tcgata
//
